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KMID : 0603820090150040309
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 4 p.309 ~ p.317
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Analysis of Protease and Antiprotease Concentrations in Retired Workers Exposed to Inorganic Dusts
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Shin Jae-Hoon
Lee Jong-Seong
Kim In-Sik
Choi Byung-Soon
Hwang Joo-Hwan
Lee Kyung-Myung
Lee Jeong-Oh
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Abstract
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Occupational exposure to inorganic dusts such as coal and silica has been identified as a chronic obstructive pulmonary disease (COPD) risk factor. This risk factor causes lung inflammation and protease-antiprotease imbalance. This abnormal inflammatory response of the lung induces parenchymal tissue destruction and leads to progressive airflow limitation that is characteristics of COPD. The aim of this study was to determine the relationship of proteases such as neutrophil elastase (NE) and matrix metalloproteinase (MMP)-9 and antiproteases such as alpha-1 antitrypsin (AAT) and tissue inhibitors of metalloproteinase (TIMP)-1 with lung function. The study population contained 223 retired workers exposed to inorganic dusts. We performed lung function test, including percent of forced expiratory volume in one second (%FEV₁) predicted and %FEV₁/forced vital capacity (FVC). We analyzed serum MMP-9, AAT, TIMP-1 and plasma NE concentrations by sandwich enzyme immunoassay. NE, AAT, and TIMP-1 concentrations in workers, who had %FEV₁<80% predicted, were higher than those of workers who had %FEV₁ ≥80% (P<0.05). Both AAT and TIMP-1 concentrations in workers with airflow limitation were higher than those of workers with normal airflow (P<0.05). %FEV₁ predicted showed significant negative correlation with AAT (r=-0.255, P<0.01), TIMP-1 (r=-0.232, P<0.01), and NE (r=-0.196, P<0.01). %FEV₁/FVC predicted showed significant negative correlation with NE (r=-0.172, P<0.05). From the results of stepwise multiple regression analysis about %FEV₁ and %FEV₁/FVC, significant independents were NE (r=-0.135, P=0.001) and AAT (r=-0.100, P=0.013) in %FEV₁, and NE (r=-0.160, P=0.014) in %FEV₁/FVC. In the present study, there were significant correlations between airflow limitation and protease concentration and between airflow limitation and antiprotease concentration. Serum protease and antiprotease concentrations, however, may be affected by the biological and inflammatory responses. It is necessary to evaluate specimens more reflected the effects of proteases and antiproteases in the lung such as lung tissue, bronchoalveolar lavage fluid, and exhaled breath condensate (EBC).
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KEYWORD
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Chronic obstructive pulmonary disease(COPD), Alpha-1 antitrypsin deficiency(AATD), Neutrophil elastase(NE), Matrix metalloproteinase (MMP), Tissue inhibitors of metalloproteinase(TIMP)
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